ZFIN Image: Jing et al., 2013, Fig. 3

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Figure Caption

Fig. 3 Identify plcg1 is indeed responsible for phenotypes of ldd239. ((A)–(F)) Plcg1 mRNA can rescue the ldd239 defect. Injection of plcg1 mRNA can restore the decreased mpx expression in mutant ICM region at 22 hpf compared with mutants ((C) and (D), black arrows). Simultaneously, deficient angiogenesis can also be rescued by overexpression of plcg1 ((E) and (F), black arrows). ((G) and (H), (K) and (L)) Plcg1 morphants have the same phenotypes as ldd239 mutants. Plcg1 morphants have a decreased mpx expression like ldd239 mutants ((G) and (H), black arrows) and also have a deficient angiogenesis marked by flk1 deficient expression ((K) and (L), black arrows). ((I) and (J)) WISH for l-plastin. Expression of l-plastin ((I) and (J)) has no change at 22 hpf in plcg1 morphants.

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Phenotype details

Acknowledgments

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Reprinted from Developmental Biology, 374(1), Jing, C.B., Chen, Y., Dong, M., Peng, X.L., Jia, X.E., Gao, L., Ma, K., Deng, M., Liu, T.X., Zon, L.I., Zhu, J., Zhou, Y., and Zhou, Y., Phospholipase c gamma-1 is required for granulocyte maturation in zebrafish, 24-31, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.