ZFIN Image: Ye et al., 2013, Fig. S4

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Figure Caption

Fig. S4 Defects in S1pr2/Gα₁₃ signaling do not induce apoptosis in the endoderm. Whole-mount TUNEL assay was performed in 17 hpf (16-somite stage) Tg(sox17:EGFP) control embryos (A-A′, n=15 ) and embryos injected with the s1pr2/mil MO (B-B′, n=15) or gnb13ab MOs (C-C′, n=15) using an apoptosis detection kit (ApopTag Red in situ apoptosis Detection Kit, Millipore) according to the manufacturer’s instructions. Rhodamine-labeled apoptotic cells (red) are not detected in the EGFP-expressing endoderm. (A-C) Lateral view with anterior towards the left; (A′-C′) dorsoanterior view with anterior upwards. Scale bars: 100 μm.

Acknowledgments

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