IMAGE

Fig. 8

ID
ZDB-IMAGE-130311-14
Source
Figures for Ye et al., 2013
Image
Figure Caption

Fig. 8 S1pr2/Gα13 signaling functions autonomously within the endoderm to regulate myocardial migration. (A) The cell transplantation procedures. Sox32-overexpressing and rhodamine-dextran labeled wild-type or gna13ab or s1pr2 morphant donor cells were transplanted into Tg(sox17:EGFP)/(myl7:EGFP) host embryos. In the case of hosts to be injected with wild-type donor cells, the embryos were injected with gna13ab MOs. (B-G′′) Epifluorescence images of the anterior region of Tg(sox17:EGFP)/(myl7:EGFP) hosts at 26-28 hpf, showing the morphology of the anterior endoderm and cardiomyocytes. (B-C′′) Wild-type donor cells were transplanted into gna13ab morphant hosts. (D-E′′) gna13ab morphant donor cells were transplanted into wild-type hosts. (F-G′′) s1pr2 morphant donor cells were transplanted into wild-type hosts. (B-G) Rhodamine-dextran labeling donor cells. (B′-G′) GFP-expressing anterior endoderm and cardiomyocytes. (B′′-G′′) Merged images of B′-G′ and B-G. Dorsoanterior view with anterior upwards; yellow dots indicate cardiomyocytes; white asterisks indicate endodermal holes; the number of embryos is indicated at the bottom left of the ‘donor’ panel; average endodermal width is shown at the bottom right of the ‘merge’ panel; #P<0.001 versus the corresponding control groups. Scale bars: 100 μm.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development