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Fig. 2 Immunohistochemical staining of skeletal muscle biopsy from the back side of zebrafish with MAb Tit1 5 H1.1. (A) Cryoslice of zebrafish skeletal muscle immunostained with MAb Tit1 5 H1.1, fixed with 4% PFA, specific staining (green) with Alexa 488, obj.100x. (B) Mechanically separated skeletal muscle fibre of zebrafish, fixed with 4% PFA, immunostained with MAb Tit1 5 H1.1, specific staining (red) with Alexa 594, obj.100x. Slices were embedded with Prolong Gold anti-fade reagent.
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