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Fig. 1 Rap1 knock-down led to abnormal heart and caudal fin development in zebrafish.
Ubiquitous expressions of (A) rap1a and (B) rap1b were shown at 24 hpf. rap1MO injection led to the heart and caudal fin-fold phenotypes and curved trunk (arrows in C) at 48 hpf compared to wild type fish, and rap1 mRNA partially rescued the heart malformation in rap1 animals (C). The statistics indicates the proportion of fish embryos with heart phenotype was significantly decreased by rap1 mRNA rescue (D). mp157e fish was used to show the heart morphology of wild type, morphant and rescued morphant at 48 hpf at a higher magnification, the atrium (red), ventricle (green) and cardiocoelom (white) are marked by color dotted lines (E). In rap1MO larvae, the heart apparently failed to develop properly, while rap1 mRNAs partially restored the heart looping failure. Scale bar, 150 µm in A, B; 250 µm in C; 100 µm in E. Columns and error bars in D show mean±S.D. n>300 in each group.