ZFIN Image: Wang et al., 2013, Fig. 3

Image

Figure Caption

Fig. 3 Fusion of Wdr68 to the Mad repression domain is not tolerated in vivo.

A-E) Ventral views of alcian blue stained animals with the Meckel’s (M), palatoquadrate (PQ) and ceratohyal (CH) labeled in red. A) Wildtype sibling. Red arrow indicates the M-PQ jaw joint. B) Severe craniofacial defects in wdr68^hi3812 animals and failure of GFP mRNA to rescue. C) Rescue of the severe craniofacial defects by injection of FlagWdr68 (FW) mRNA. Red arrow indicates the mild M-PQ joint fusion phenotype. D) Severe craniofacial defects in wdr68^hi3812 animals and failure of MadFlagWdr68 (MFW) mRNA to rescue. 52/52 mutants genotyped were wdr68^hi3812 homozygotes. E) Rescue of the severe craniofacial defects by injection of CebpFlagWdr68 (CFW) mRNA. Red arrow indicates the mild M-PQ joint fusion phenotype. F) Graph summarizing the results of four injection trials. In aggregate, n = 112/452 (25%) for GFP animals, n = 16/352 (4.5%) for FW animals, n = 96/296 (32%) for MFW animals, and n = 2/149 (1.3%) for CFW animals.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One