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Fig. S1 auts2 expression and morphant phenotype. (A) Whole-mount in situ hybridization of auts2 shows that it is expressed in the forebrain (fb) (including olfactory organs), midbrain (mb), hindbrain (hb), spinal cord (sc), the caudal peduncle and eye at 24hpf. At 48hpf, auts2 is expressed in the brain, pectoral fin and eye. At 120 hpf expression is restricted to the brain, primarily the midbrain, and weakly in the eye. (B) Fish injected with the 5 bp translational MO mismatch control have indistinguishable morphology as wild type fish at 24, 48 and 120 hpf. (C) Injection of the auts2 translational MO results in fish with a stunted development phenotype that includes smaller heads, eyes, bodies and fins. (E) Injection of the auts2 splice-blocking MO shows a similar but less severe phenotype than the auts2 translational MO. (E) The auts2 translational MO phenotype is partially rescued by co-injecting the full length human AUTS2. Note the longer body and larger brain compared to the translational and splicing morphant fish. MO injected fish in C, D, and E are scaled to the 5 bp injected control fish in B.