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Fig. 8 Expression of oxtl mRNA is induced in the hindbrain by exposure to ethanol.
Whole mount in situ hybridization was used to detect: A, GFP; B, oxtl; and C, tyrosine hydroxylase (th) mRNAs in Tg(oxtl:GFP) larva treated with ethanol for 24 hrs at 4 dpf, and then fixed at 6 dpf. th labels dopaminergic and noradrenergic neurons located in the area postrema. Examples of positive staining are indicated by arrows.
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