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Fig. 2 Mpi knockdown decreases full-length LLO and mannose-6-phosphate levels in zebrafish. (A) Full-length LLO (G3M9) levels are decreased by Mpi knockdown, as seen on FACE analysis. (B) G3M9 LLO (normalized to number of fish) and mannose-6-phosphate (M6P) levels (normalized to 100 microgram protein) in lysates from 4 dpf zebrafish larvae. Values from larvae injected with the standard control morpholino were set as 100%. Average from three experiments. (C) N-glycans are decreased to 53% in mpi morphants on FACE analysis as compared with standard controls (n=2 experiments).
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Full text @ Dis. Model. Mech.