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Fig. 5 The sdf1a expression pattern is disturbed in gata4 morphants, while quantitative analysis confirms changes in expression in morphant embryos for sdf1a and hemato-vascular markers.
Shown are representative embryos (n >50) following in situ hybridization to detect expression of sdf1a transcripts in control (A,C) and morphant (B,D) embryos, at 30 hpf (A,B) and 35 hpf (C,D). In panels A and B, 2 independent embryos are shown representing the range of patterns detected. Red arrows pointing down show the strong stripes of expression in lateral mesoderm in control embryos, which is nearly gone by 35 hpf. In contrast, the pattern is less clear in mesoderm of 30 hpf morphant embryos, but strongly persists by 35 hpf, and is much enhanced in the region associated with the pronephric tubules, particularly at 35 hpf (black arrows pointing up in panels B and D). Views are lateral, anterior to the left. (E) Tails including the CHT region were isolated from control and gata4 morphant embryos at 40, 48, or 72 hpf and processed by qPCR to measure relative levels of ae3globin, runx1, lmo2, cmyb, and sdf1a. The Y axis plots the relative transcript levels of morphants compared to controls (controls set at a value of 1). Each sample included >30 embryos, and data was collected in 3 independent experiments. Student’s T-test was used to determine significance (*p<0.05, **p<0.01, ***p<0.001).