|
Fig. 1 Sim1a and Arnt2 act synergistically during longitudinal HTS axon guidance. Dorsal views of hindbrain confocal z-projections of otpb:gfp transgenic embryos at 72 hpf. Anterior is towards the left. (A-H) Mediolateral positioning of otpb:gfp axons (arrows) after indicated treatments. otpb:gfp hindbrain neurons (asterisks in A,C,E,G) do not contribute to longitudinal projections. (I) Quantification of the distance between otpb:gfp axons (I), of the distance between MA neurons (II) or between otpb:gfp hindbrain neurons (III). *P<0.0001 for sim1a MO 1.0 ng, arnt2 MO 4.5 ng, arnt2/sim1a MO 1.0/4.5 ng versus control MO 4.5 ng. Scale bar: 50 μm.