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Fig. 2 etv5b-deficient embryos exhibit fewer 5-HT neurons in the hypothalamus. (A-J) Confocal maximum intensity projections showing controls and etv5b morphants processed for 5-HT immunohistochemistry. The intermediate/posterior (i./p.) clusters of the hypothalamus (A,B,E-G) and the 5-HT population of the anterior raphe (r.) are indicated (C,D,H-J). Ventral views, anterior left. (K-T) nkx2.1a expression in the basal forebrain. Lateral (K,L,O-Q) and ventral (M,N,R-T) views, anterior left. (U,V) The number of 5-HT cells at 72 hpf in controls and etv5b ATG (0.075 mM, U) or splice (0.15 mM, V) morphants expressed as percentage of control. n, total number of embryos analysed for each experiment. *Pd0.05; n.s., not significant. Scale bars: 50 μm.