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Fig. 7 Transgenic islet2 expression in slg mutants rescues the axon outgrowth defect. (A) An mnx1-3×125bp enhancer sequence (mnx1) was used to direct protein co-expression in the same primary motoneurons (PMNs) from two injected plasmids (mRFP and GFP). (B) Expression of Prdm14 or Islet2 in CaP rescues the axon outgrowth defect of slg mutant zebrafish embryos. Arrowheads indicate CaP cell bodies. (C) Summary of results in B. n, number of RFP-labeled CaP neurons. n=41 from 20 injected embryos in the mnx:RFP group (control); n=53 from 33 injected embryos in the mnx:RFP + mnx:prdm14 group (prdm14); n=49 from 28 injected embryos in the mnx:RFP + mnx:islet2 group (islet2). (D) Proposed regulation of CaP axon outgrowth. Prdm14 regulates CaP axon outgrowth through islet2 and as yet unidentified factors (question mark). Scale bars: 20 μm.