Fig. 3

Fig. 3 Morpholino-mediated inactivation of zebrafish atxn7 impairs embryonic development.

Schematic representation of the zebrafish atxn7 gene showing exons 1 (ex 1), 3 to 5 (ex 3-5) and 12 (ex 12) (black boxes), location of MOzatxn7^AUG and MOzatxn7^SPL (red lines) and position of oligonucleotides (black arrows) used for RT-PCR analysis of MOzatxn7^SPL-mediated inhibition of zebrafish atxn7 intron 4 splicing (black arrows) (A). Untranslated exonic regions and intronic sequences are depicted as empty boxes and single lines, respectively. RT-PCR analysis of zebrafish atxn7 intron 4 splicing in non-injected (NI) and morphant embryos that had received 0.3, 0.6 and 1 pmol MOzatxn7^SPL (B). RT-PCR for β-actin is shown as a positive control. Phenotypes of 48 hpf wild-type embryo (C), and age-matched 1 pmol mmMOzatxn7^AUG (D), 0.3 pmol MOzatxn7^AUG (G) and 0.3 pmol MOzatxn7^SPL morphants (H). Phenotypes of 24 hpf 1 pmol MOzatxn7^AUG (E) and 1 pmol MOzatxn7^SPL morphants (F).