Fig. 5

Fig. 5

Mosaic BMP Loss of Function at Early Stages inside the Telencephalon Results in Ectopic Expression of rx3 Embryos were injected with HSP70:dnBMPr plasmid at one- to two-cell stage followed by heat shock at oblong stage/early blastula (A–L) and shield stage/early gastrulation (M and N), respectively. The arrows in (A)–(H) mark cells with ectopic rx3 expression at stages indicated. (E and F) Close-up of the boxed area marked in the inset. (I–L) Expression of telencephalic (Tel.) markers, tlc and emx3, is maintained in dnBMPr-expressing cells (arrows in I–L). (M and N) Heat shock at 50% epiboly/early gastrulation does not result in ectopic rx3 expression (arrows in M and N). All views are dorsal, anterior at the top. White dotted lines in (C), (D), (M), and (N) mark the border of the eye field. All figures are z-projections of confocal sections. 1s, one-somite stage.