ZFIN Image: Shin et al., 2012, Fig. 5

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Figure Caption

Fig. 5 nf1a/nf1b mutants exhibit upregulation of pERK1/2. (A) Western blot analysis for pERK1/2 in protein lysates prepared from wild-type, nf1a^-/-; nf1b^+/+, nf1a^+/+; nf1b^-/- and nf1a^-/-; nf1b^-/- larvae (100 μg) reveals increased pERK1/2 levels in nf1a^-/-; nf1b^-/- larvae as compared with wild-type, nf1a^-/-; nf1b^+/+ and nf1a^+/+; nf1b^-/- larvae at 3 dpf. Equal loading was confirmed by stripping the membrane and reprobing for total ERK1/2. (B-Q) Transverse spinal cord sections of wild-type (B,F,J,N), nf1a^+/-; nf1b^-/- (C,G,K,O), nf1a^-/-; nf1b^+/- (D,H,L,P) and nf1a^-/-; nf1b^-/- (E,I,M,Q) larvae labeled with anti-pERK1/2 antibody (B–E, green), anti-pS6 antibody (F-I, red), or anti-HuC/D antibody (J-M, magenta) demonstrate marked upregulation of pERK1/2 in nf1a^-/-; nf1b^-/-larvae and intermediate levels of pERK1/2 in larvae harboring a single functioning nf1 allele as compared with wild-type larvae at 4 dpf (n=5 each for wild-type and mutant larvae). Scale bars: 40 μm.

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Acknowledgments

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