ZFIN Image: Yabe et al., 2012, Fig. 3

Image

Figure Caption

Fig. 3 msgn1 expression driven by the 3000-bp msgn1 promoter rescues msgn1 morphant embryos from the PSM phenotype. (A) Schematic representation of DNA construct used in the experiments. The msgn1 ORF was connected to the C-terminus of mCherry via the 2a peptide. The translation of mRNA transcripts from this cDNA is expected not to be inhibited by msgn1 MO, because this MO blocks the translation initiated from the start codon of msgn1 mRNA. This construct was inserted between the tol2-inverted repeats following the 3000-bp msgn1 promoter. (B)-(E) The anterior migration of mCherry-positive cells and tbx24 expression in msgn1;spt double-deficient embryos were rescued by the injection of 25 pg of rescue construct with 50 pg of synthesized tol2 transposase mRNA (D and E, n=48, 100%) but not by injection of the construct without the msgn1 ORF (B and C, n=48, 100%).

Acknowledgments

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Reprinted from Developmental Biology, 370(2), Yabe, T., and Takada, S., Mesogenin causes embryonic mesoderm progenitors to differentiate during development of zebrafish tail somites, 213-222, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.