Fig. S4

Fig. S4 Isolation of msgn1 promoter. Schematic representation of msgn1 promoter isolation. An approx. 3000-bps genomic fragment upstream of then msgn1 coding region was isolated by ScaI and AccIII digestion of a Bac clone. This sequence was fused to the 5′ of mCherry and cloned between the tol2 inverted repeat elements. (B-D) The expression of mCherry in transgenic embryos. The embryos carrying the transgene containing mcherry connected to the down-stream of the msgn1 3000-bp promoter recaptured the endogenous msgn1 expression in the tailbud and the posterior PSM. This DNA element also drove the expression in the anterior paraxial mesoderm and somites, possibly due to the lack of some cis-element required to terminate the transcription at the anterior paraxial mesoderm or because of a difference of mRNA stability between msgn1 and mcherry (B and C). The mCherry protein was also detected in the paraxial mesoderm (D).