ZFIN Image: Tsetskhladze et al., 2012, Fig. 2

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Figure Caption

Fig. 2 Effect of human coding polymorphisms on zebrafish mRNA rescue of the albino phenotype.

Lateral views of 48-hpf (A) wild-type zebrafish larva (B) un-injected alb^nk1 zebrafish larva (C-F) alb^nk1 zebrafish larva injected with mRNA (500 pg), coding for indicated variants of zebrafish slc45a2, named according to positions of human variation (human 272 equivalent to zebrafish 303; human 374 equivalent to zebrafish 403). (C) wild-type; (D) E272K mutant; (E) L374F mutant; (F) E272K/L374F double mutant. Note that mRNA rescue in zebrafish does not occur in every cell, which is thought to be due to unequal distribution of the mRNA in the cytoplasm of the originally injected eggs, resulting in unequal distribution of the mRNA among different cells of the embryo. The results shown are typical of a majority of injected embryos in each case; each of embryo in the majority populations contains cells as pigmented as the ones evident in this figure. Scale bar 400, μm.

Figure Data

Phenotype details

Acknowledgments

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