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Fig. S1 Effects of emi1 and cdh1 morpholinos on RNA splicing and phosphorylation of Histone H2AX (pH2AX). (A) RT-PCR analysis of RNA from pools of 20 zebrafish embryos following injection with mismatch control (C), emi1 or cdh1 morpholinos as indicated. The gene for which RT-PCR was performed is indicated to the left of the panels. The aberrant RT-PCR products indicated were subcloned and verified by sequencing. Both morpholinos were designed to target the exon 2– intron 2 splice-junction and caused deletion of exon 2 (indicated by “del”) and/or partial (“part”) or total insertion of intron 2. RT-PCR of beta (β)-actin is a control for RNA quality and quantity. (B) Phospho-Histone H2AX (pH2AX) Western analysis of lysates from pools of embryos injected with the indicated morpholinos. Note the increased amount of pH2AX in emi1 morphants, which is rescued back to normal levels by cdh1 knock down.