Fig. S1

Fig. S1 celf1 plays a crucial role in symmetric somitogenesis and LR asymmetric patterning. (A) celf1_long-MO and celf1_short-MO inhibited the expression of celf1L-GFP and celf1S-GFP, respectively. (B) Western blotting using anti-Celf1 antiserum. Left panel: co-injection of celf1_long-MO with celf1_short-MO (celf1-MOs) inhibited the expression of both forms of endogenous Celf1 in zebrafish embryos. The asterisk indicates a non-specific band. Right panel: Celf1 and Celf1^Δlinker proteins were detected in Celf1- and Celf1^Δlinker_overexpressing embryos, respectively. (C) Representative images of myod expression demonstrating normal (left; control-MO, n=105) or abnormal (right; celf1-MOs, n=88) somitogenesis in embryos at 24-28 hpf. Higher magnification images (lower panels) highlight somites. Knockdown of celf1 resulted in a mild loss of the chevron shape of somites (94%). (D) Upregulation of myod expression in celf1 morphants. qPCR assay revealed that celf1 knockdown resulted in a 50% increase of myod expression in zebrafish embryos, suggesting that myod is a target of Celf1 not only in C2C12 (Lee et al., 2010) but also in zebrafish.