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Fig. 5 Effects of vitamin D receptor (VDR)a and VDRb morpholino oligonucleotides (MOs) in 3-d post-fertilization (dpf) zebrafish embryos.
Ca2+ influx (A), Ca2+ content (B), mRNA expression (C), density of ecac-expressing cells (D), and ecac signals (E). mRNA expressions were analyzed by a qPCR using β-actin as the internal control. Different letters indicate a significant difference (p<0.05) using a one-way ANOVA followed by Tukey’s multiple-comparison test. Values are the mean ± SD (n = 6 or 7). Scale bar:100 μm.
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