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Fig. S4 Disruption of Ca2+ signaling affects dorsal specification. (A,D) Dorsal nuclear accumulation β-Catenin in DMSO-treated embryos (yellow arrowheads in A) in comparison with absence of β-Catenin in thapsigargin-treated embryos (D). (B,C,E,F) DAPI staining (B,E) shows all nuclei in control and thapsigargintreated embryos, and C,F show merged images for β-Catenin and DAPI staining. Scale bars: 50 μm.
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