Fig. S5

Fig. S5 Additional evidence for hepatocyte-specific inactivation of supv3l1 in supv3l1^GtN/GtN, hepatocyte-Cre fish. (A) No mCherry was detected in 18-d-old supv3l1^GtN/GtN fish, including in the liver. (B) mCherry was detected only in hepatocytes of 18-d-old supv3l1^GtN/GtN, hepatocyte-Cre fish, but not in the 2F11+ ductal cells or in intestinal cells. GL, gut lumen; L, liver; K, kidney head. (C) Relative levels of supv3l1 transcripts in the liver of 18-d-old supv3l1^GtN/GtN and supv3l1^GtN/GtN, hepatocyte-Cre fish were determined by RT-PCR. Error bar = SEM. (D) Relative levels of supv3l1^GtT allele and supv3l1^GtN allele in the liver of 30-dold LiPan+//hepatocyte-Cre+// supv3l1^GtNf/+ and LiPan/supv3l1^LKO fish were determined by qPCR. Error bar = SD.