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Fig. S1 Gene-trap cassette optimization. (A) Schematic of the mutagenicity reporters for candidate gene-trap cassettes. (B) Mutagenicity assay by semiquantitative RT-PCR analysis indicated that a cassette with three copies of BGH pA is more mutagenic than one with one copy. (C) Semiquantitative RT-PCR analysis indicated that a cassette with five copies of BGH pA is more mutagenic than one with three copies.
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