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Fig. 1
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Fig. 1
mRNA expression analysis of zebrafish membrane tethered mmps. RT-PCR was used to amplify mmp cDNA sequences generated from 1 µg total RNA collected at multiple embryonic stages. PCR was performed for 35 cycles because 40 cycles amplified saturating amounts of DNA product. Equal volumes of PCR product were loaded onto an agarose gel and detected using ethidium bromide and UV transillumination. To detect maternal mmp gene expression, total RNA was isolated from <8-cell stage embryos. hpf, hours post-fertilization; TB, tailbud.
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Reprinted from Gene expression patterns : GEP, 12(7-8), Quick, R.E., Dunlap, J.A., and Jessen, J.R., Expression analysis of zebrafish membrane type-2 matrix metalloproteinases during embryonic development, 254-260, Copyright (2012) with permission from Elsevier. Full text @ Gene Expr. Patterns