ZFIN Image: An et al., 2012, Fig. 3

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Figure Caption

Fig. 3 Molecular consequences of sf3b1 mutations (A,B).Variant transcripts of sf3b1 in tst^b460 mutants. (A) RT-PCR products were amplified between exon1 and exon6 of sf3b1 with 20-24 hpf AB wildtype and tst^b460 homozygous mutant total RNA as template using Sf3b1aa (46)F and Sf3b1aa (579)R primers. The single AB wildtype RT-PCR product was 534 bp (tran1). tst^b460 homozygous mutants contained 4 different transcripts; tran1(534 bp), and variant transcripts tran2(412 bp), tran3(390 bp), and tran4(312 bp). (B) Diagram of variant transcripts in tst^b460 homozygous mutants. (C) Western blot analysis of Sf3b1 protein levels in wildtype, tst^b460 mutant and hi3394a mutant embryos at 24 hpf. Lane 1: wildtype embryos; Lane 2: tst^b460 homozygous mutants; Lane 3: hi3394a homozygous mutants; Lane 4: hsp>sf3b1 injected tst^b460 mutants with melanophore phenotype rescue.

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Acknowledgments

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