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Fig. S4
Characterization of Igsf11 antiserum. (A) Knockdown by morpholino oligonucleotide injection reduced Igsf11 immunoreactivity in embryos at 24 hours post-fertilization. Igsf11 immunoreactivity was present along vertical myosepta in uninjected embryos as well as embryos injected with a control igsf11 5 bp mismatch morpholino (igsf11-MM), but was dramatically reduced in embryos injected with a morpholino targeting the igsf11 translational start site (igsf11-MO). Embryos were injected with 4 ng of either morpholino and exposure times were identical for all images shown. (B,C) In addition to scattered cells in the hypodermis and extra-hypodermal locations (main text), both in situ hybridization (B) and immunohistochemistry (C) revealed igsf11-expressing cells (arrowheads) in the spinal cord during the larval-to-adult transformation (larvae shown here at ~9 SSL [11]). Staining appears more extensive in B than C owing to different section thicknesses (150 µm, 20 µm, respectively).