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Fig. S14 TUNEL staining of developing zebrafish midbrain. (A) TUNEL staining of DNase I-treated Tg(kdrl:eGFP) zebrafish brain at 3 dpf. DNase I treatment generates strand breaks in the DNA to provide a positive TUNEL reaction. Red, TUNEL signal. The dashed white line delineates the outline of midbrain. (B) Staining of Tg(kdrl:eGFP) zebrafish larva brain without terminal transferase, serving as a negative control. (C and D) Two examples of TUNEL staining of Tg(kdrl:eGFP) zebrafish brain (WT1, WT2) at 3 dpf. Scale, 50 μm.
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