Fig. S16 Effects of DMOG treatment on vessel pruning. (A) o-Dianisidine staining showing that DMOG treatment increases the amount of blood cells in treated embryos. DMSO (0.2%) or DMOG (0.2 mM) was bath-applied during 2?3 dpf. (B and C) Effect of DMOG treatment on vessel pruning of larval zebrafish midbrain. Projected confocal images showing midbrain vasculature of DMSO- (B) and DMOG-treated (C) zebrafish larvae at 3 dpf. (D) Average number of vessel pruning events occurring between 2 and 3 dpf in single larval zebrafish midbrain. Each small square in (D) represents the data obtained from single larvae. Scale, 50 μm. n.s., no significance (Student′s t test). Error bars, ± SEM.
Image
Figure Caption
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ PLoS Biol.