ZFIN Image: Liu et al., 2012, Fig. 3

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Figure Caption

Fig. 3 Loss of integrin α_v, integrin β₈ or combinatorial knockdown of both genes results in hemorrhage. As compared to uninjected controls in (A), 12 ng of itgav^e3i3 MO (B), or 4 ng of itgb8^e2i2 MO (C), leads to head hemorrhages at 50–52 hpf. (D–F) Suboptimal morpholino doses (3 ng of itgav^e3i3 MO (D) or 1 ng of itgb8^e2i2 (E)) results in small hemorrhages while double knockdown of both genes using these low doses results in numerous, larger hemorrhages (F, arrows). (G) Graphical representation of hemorrhage rate as a function of the dose of morpholino injected. Percentage of embryos with hemorrhages is shown above the bars. # represents the numbers of embryos scored for each analysis.

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Acknowledgments

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Reprinted from Developmental Biology, 363(1), Liu, J., Zeng, L., Kennedy, R.M., Gruenig, N.M., and Childs, S.J., betaPix plays a dual role in cerebral vascular stability and angiogenesis, and interacts with integrin alphavbeta8, 95-105, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.