Fig. 2

Fig. 2 tbl3is specifically expressed in wild-type and lost inceymutants. Mapping the cey lesion revealed a telomeric deletion on chromosome 3 (A, not to scale). Double dashed lines indicate the approximate location of the deletion. Z-markers are listed on the left of the chromosome schematic and genes are on the right. The gene sox9b is excluded from the deletion (B), while tbl3 is included in the deletion (C). Radiation hybrid mapping indicates that tbl3 is located on the very end of the telomere on chromosome 3 and is closely linked to the noxo1a (GenBank ID: NM_001077584.1) gene (A). tbl3 is maternally expressed at the 8-cell stage (D). By 19.5 hpf tbl3 is still widely expressed in most tissues with high expression in the eye (E). tbl3 is expressed in most tissues between 29 and 30 hpf (F). At 35 hpf tbl3 expression is high in the eye and brain (E) and by 46–48 hpf (F) expression is present in the intestinal region (green arrowhead) and the HSPCs (inset—larvae in inset is different than larvae in the panel). Cross sections confirm that tbl3 is expressed between the dorsal aorta (red asterisk) and the caudal vein (yellow asterisk) at 45–48 hpf where HSPCs are found (I). Cross sections of the retina at 45–48 hpf (J) and 75 hpf (K) show that tbl3 is expressed around the lens (arrow) and in the retinal stem cell region, also known as the ciliary marginal zone (CMZ; asterisks) at the lateral edge of the retina adjacent to the lens. At 75 hpf (K) tbl3 is also expressed in the inner nuclear layer (bracket). tbl3 expression remains high in the eye (black), brain, fin bud (blue), and gut (green) at 75 hpf (L, M). Consistent with the loss of the tbl3 gene in cey, tbl3 expression is absent in cey mutant embryos (N, O). Accession numbers for other genes on the chromosome in (A) are as follows: foxj1a (GenBank ID: NM_001076706.2), zgc:152997 (GenBank ID: NM_001077319.1), sox8 (GenBank ID: NM_001025465.1), and zgc:113100 (GenBank ID: NM_001031844.1). Scale bars are 60 μm (I), 130 μm (D–H, J–O), and 245 μm (N, O).(For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)