ZFIN Image: Feng et al., 2012, Fig. 3

Image

Figure Caption

Fig. 3

Leukocyte Depletion Prevents V12RAS⁺ Cell Growth, which Can Be Partially Rescued by dmPGE₂(A–I) Representative images of flank skin regions illustrating numbers of V12RASeGFP⁺ transformed cells after morpholino (Mo) knockdown of pu.1 and gcsfr (for depletion of both neutrophils and macrophages); gcsfr alone (for depletion of neutrophils); irf8 or a lower dose (2 nl/embryo) of pu.1 morpholino alone (for depletion of macrophages) with or without additional treatment with dmPGE_2.(J) Graphic illustration of how dmPGE₂ can rescue the number of transformed cells in morphants in which both neutrophils and macrophages have been specifically depleted (p < 0.01, n = 33, 32, and 34).(K) Graphic illustration of how dmPGE₂ can only partially rescue morpholino knockdowns that largely target neutrophils (p < 0.001, n = 25, 25, and 22).(L and M) Similar graphic illustration of how dmPGE₂ can rescue morpholino knockdowns that largely target macrophages (irf8 Mo-p < 0.05, n = 24, 26, and 23; 2 nl pu.1 Mo-p < 0.05, n = 31, 30, and 34).The scale bar represents 100 µm. See also Figures S3 and S4.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.