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Fig. S4 Injection of hsp70-dTomato-pax2a plasmid induces robust Pax2a expression following heat-shock. (A-A2′) Embryos injected with a heat-shock-inducible construct expressing both Pax2a and the dTomato reporter were heat-shocked at 12hpf. These embryos display mosaic Pax2a expression 3 hours post-heat-shock. Arrows indicate cells with ectopic Pax2a expression. (B) Quantification of induced Pax2a levels relative to endogenous Pax2a. Note that on average ectopic Pax2a levels are 2.9-fold higher than the endogenous levels (n=42 cells from five embryos; Student’s t-test, ***P<<0.001). Error bars represent s.e.m. (C) Distribution of Pax2a fluorescence intensity comparing endogenous Pax2a with ectopically induced Pax2a. Note that overall intensity levels of the exogenous Pax2a fall within a high endogenous spectrum (n=198 cells from five embryos). Scale bar: 50 μm.