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Figure Caption
Fig. S9 ?ENotch1a localizes to the apical region, but does not alter apical domain size. (A) Comparison in the localization of membrane associated GFP-CAAX versus signal sequence tagged (s)-GFP-?ENotch1a. Both transgenes were driven by a UAS promoter and injected into vsx2:gal4 transgenic fish (Clark et al., 2011). Note the apical enrichment at the plasma membrane and in intracellular puncta of sGFP-?ENotch1a. (B) Average apical domain size in GFP-CAAX versus sGFP-DENotch1a. No significant difference between groups; P=0.51, Students t-test.
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