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Fig. S4 Functional characterisation of the facial lymphatics. (A-A′′) Lateral images of the lyve1:DsRed2 transgenic at 8 dpf showing the FL (A), its dye uptake a few minutes after subcutaneous injection of high molecular weight fluorescein dextran (n=10) (A′), and the merged image of A and A′ (A′′). (B-B′′) Lateral images of lyve1 (B), gata1 (B′) and merged (B′′) expression of the lyve1:EGFP:gata1:DsRed transgenic at 5 dpf showing no blood flow in the FL. (C) The lack of blood flow is more clear in ventral confocal image of the lyve1:EGFP:gata1:DsRed transgenic at 5 dpf. (B-C) Montage images of two z series stacks. FL, facial lymphatics. Scale bars: 100 μm.