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Fig. 2 dlx3b-expressing olfactory placode (OP) precursors share a common border with cranial neural crest cells (CNCCs). Visualization of OP convergence (using dlx3b in situ hybridization, in blue) and the CNC field (using anti-green fluorescent protein [GFP] immunocytochemistry, in brown), in sox10:EGFP embryos. A–I: Dorsal views, rostral to the top of the page, of fixed-staged, double-labeled embryos. Embryos were examined every 2 somites. E,E′: Two different focal planes of the same embryo at 12s (somite stage): E, dorsal olfactory placode; E′, ventral edge of the olfactory placode. F,F′: Two different focal planes at 14s: F, dorsal olfactory placode; F′, ventral edge of the olfactory placode. The CNCCs are both dorsal and ventral to the olfactory fields at these stages. J,K: Ventral views (rostral to the top) of the formed olfactory placode surrounded by CNCCs at 18s (J) and 20s (K). Scale bars = 30 μm in A (applies in A–K). Twenty embryos were examined per time point.