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Fig. S2
Craniofacial structures after PD0325901 treatments. A. Craniofacial structures of the 96 hpf zebrafish stained with Alcian blue after administration of increasing concentrations of PD0325901 (0.1-1.0 μM) from 4 hpf. Craniofacial cartilages were dissected from the body and additional tissues removed for imaging. Meckel?s cartilage (orange arrowheads), ceratohyal cartilage (black arrowheads), and pharyngeal arches (red arrowheads) are indicated. B. Comparison of craniofacial cartilage of 4 day old control embryos (DMSO) and embryos treated with 1 μM PD0325901 added at the indicated timepoints in development (4 - 72 hpf). Meckel?s cartilage (orange arrowheads) and ceratohyal cartilage (black arrowheads) are indicated. The numbers of embryos displaying the presented phenotype are indicated. C. Simplified, illustrative comparison of 4 dpf zebrafish jaw structures after control or intensive PD0325901 treatments 4 hpf (ventral view). High concentrations of the MEK inhibitor cause structural malformation, distortion of the angle between Meckel?s and ceratohyal cartilages and complete lack of the 4th and 5th branchial arches. The seven pharyngeal arches represented are: MC: Meckel?s cartilage (light blue); CH: ceratohyal cartilage (dark blue); 1st-5th branchial arches (green), fins (grey).