Fig. 5 Expression profile of the zebrafish 5-HT2C receptor gene in different tissues generated by RT-PCR. A 310 bp fragment of the gene can be amplified from cDNA of zebrafish brain, skin, ovary, and axial muscle. The specific primers (KMXfwd/KMXrev) flank intron 4. Other tissues have not been tested. In addition, the receptor is expressed in 8 hpf and 2.5 hpf embryos. For the negative (neg) control, no cDNA was used for amplification. For the positive (pos) control, a plasmid containing the entire coding region of the receptor was used instead of cDNA. No quantitative approach was taken and the general intensity of individual fragments is not a direct indication of expression levels in individual tissues.
Reprinted from Gene, 502(2), Schneider, H., Fritzky, L., Williams, J., Heumann, C., Yochum, M., Pattar, K., Noppert, G., Mock, V., and Hawley, E., Cloning and expression of a zebrafish 5-HT(2C) receptor gene, 108-117, Copyright (2012) with permission from Elsevier. Full text @ Gene