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Fig. S4
Depletion of Ppp4c inhibits BMP2-induced expression of ID1-Luc reporter in C2C12 cells, related to Figure 4. Two independent C2C12 stable clones, Ppp4c-KD #1 and #2, were selected following transfection with Ppp4c shRNA-14. The base level and BMP2-induced ID1-Luc reporter activity in these cells was dramatically decreased in cells depleted of Ppp4c. CTRL, pSRG vector. Ppp4c knockdown effect was demonstrated by Western blot in lower panel.
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Reprinted from Developmental Cell, 22(5), Jia, S., Dai, F., Wu, D., Lin, X., Xing, C., Xue, Y., Wang, Y., Xiao, M., Wu, W., Feng, X.H., and Meng, A., Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos, 1065-1078, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell