ZFIN Image: Jia et al., 2012, Fig. S3

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Fig. S3

Ppp4cb knockdown or overexpression has little effect on Wnt target gene expression, related to Figure 3. One-cell stage embryos were injected with 1 ng ppp4cb-MO1 (bMO1) or 100 pg ppp4cb mRNA and examined by whole-mount in situ hybridization for the expression of boz at the sphere stage and vent at the shield stage. boz is a direct target of maternal Wnt signaling and vent is a target of zygotic Wnt signaling. Note that both of them were not markedly affected in down- or up-regulation of ppp4cb. Upper panel, lateral views with dorsal to the right; lower panel, animal-pole views with dorsal to the right. The ratio indicated at the right corner was the number of embryos with the phenotype shown/the number of observed embryos.

Acknowledgments

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Reprinted from Developmental Cell, 22(5), Jia, S., Dai, F., Wu, D., Lin, X., Xing, C., Xue, Y., Wang, Y., Xiao, M., Wu, W., Feng, X.H., and Meng, A., Protein Phosphatase 4 Cooperates with Smads to Promote BMP Signaling in Dorsoventral Patterning of Zebrafish Embryos, 1065-1078, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell