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Fig. 4
Maternal and ubiquitous expression of dhx8. A: Whole-mount in situ hybridization of dhx8 showing that dhx8 is maternally transcribed. Expression is ubiquitous to about 15–somite stage and then gradually becomes restricted to the brain as the embryo continues to develop. At 24–48 hpf, expression can be seen in the pronephric duct (arrow). B:dhx8 is expressed in the nucleus in a punctuate pattern. HeLa cells were transfected with GFP-dhx8 fusion constructs and observed by immunofluorescence 48 hr post-transfection.
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