Fig. S2

Fig. S2

Identification of mosaic cell populations in the fins shown in Fig. 1. (A–D) Class 1 mosaics contain labeled epidermal cells. The fin shown in Figs. 1F and F* sectioned and stained with anti-p63 antibodies (B, green). mCherry⁺ (A, red) cells are found exclusively in epidermal layers and all are p63⁺ (white arrows). (E–L) Class 2 mosaics are composed of labeled intra-ray fibroblasts. The fin shown in Figs. 1G and G* sectioned and stained with antibodies recognizing tenascin C (F, green) or zns-5 (J, green). mCherry⁺ (red) cells are in a tenascin C rich extracellular matrix (white arrows in H) but do not express the osteoblast marker zns-5 (white arrows in L). (M–P) Class 3 mosaics comprise lepidotrichia-localized osteoblasts. The fin shown in Figs. 1H and H* sectioned and stained with zns-5 antibodies (N, green). mCherry⁺ (M, red) cells in lepidotrichia are also zns-5⁺ (white arrows). For all experiments, nuclei are stained with Hoechst (C, G, K, O, blue) and the three-color overlay is shown in the last column (D, H, L, P).