Fig. S1

Fig. S1

Antibody validation. (A–D) An anti-dsRed antibody detects mCherry expression in sectioned fins. A section from a Class 1 mosaic Tg(dusp6:Cre-ERT2, EAB:EGFP-FlExmCherry) fin immunostained with an anti-dsRed antibody (A, green) with mCherry expression shown in red (B). Nuclei are stained with Hoechst (C, blue) and the overlay is shown (D). All mCherry⁺ cells are positive for dsRed (white arrows). (E–L) The zns-5 and zn-3 antibodies stain osteoblasts. Sections of non-regenerating caudal fins from Tg(sp7:EGFP) fish showing EGFP expression (E, I green) and stained with zns-5 or zn-3 antibodies (F, J, red) and Hoechst (G, K, blue). The overlays (H, L) demonstrate co-localization of EGFP⁺ cells with zns-5⁺ or zn-3⁺ cells, respectively (cells marked with white arrows).