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Fig. 3 Co-expression analysis of zebrafish prominin-1a or b with Pax-6 and/or musashi-1 within retina cell layers.
(A–D′′) Cryosections of eyes from adult zebrafish were processed for non-radioactive ISH using antisense DIG-labelled prominin-1a (A, D, D′′; prom1a), prominin-1b (B; prom1b) or musashi-1 (C; msi1) probe combined with IHC detection of Pax-6 (A′–C′, D′, D′′; anti-Pax-6). Arrowheads and arrows show the Pax-6 immunoreactivity (red) within the inner nuclear layer (INL) and ganglion cell layer (GCL), respectively. The area shown in the inset (A, A′) is displayed as zoom-in in panels D-D3. Note the expression of Pax-6 overlaps with prominin-1a within the INL. Asterisks indicate the retinal pigmented (dark brown) epithelium. Dashed lines delineate the INL. e, erythrocytes. Scale bars, 25 μm.