ZFIN Image: Arkhipova et al., 2012, Fig. S3

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Figure Caption

Fig. S3

Pax6b binding properties of the sequence motives P^1-3 and P^9-10

(A)Sequence of oligonucleotides used in the EMSA studies (top row). Indicated are the Pax6 core consensus sequences P¹, P², P³, P⁹ and P¹⁰ (blue box, letters in upper case) and nucleotides that were unchanged (line), mutated (red letters in lower case) or deleted (colon) in comparison to the genomic sequence. (B-D) Radiograms of EMSA studies with corresponding ³²P-labeled oligonucleotides (DNA, as indicated), Pax6b protein and nonlabelled competitor DNA (comp, as indicated). (B) Weak or missing signals (arrow) for P^2m+3, P^Δ1+2 and strong signals for P²⁺³, P^2+3m and P^1+2m+3 suggest that Pax6b binds with high affinity to P¹ and P² but not to P³. (C) The inefficient competition of all mutated oligonucleotides in comparison to P¹⁺²⁺³ shows that highest affinity binding requires the presence of both sites, P¹ and P². (D) Missing signals for and inefficient competition by P^9+10m show that Pax6b binds with high affinity to P¹⁰ but not to P⁹.

Acknowledgments

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Reprinted from Developmental Biology, 365(1), Arkhipova, V., Wendik, B., Devos, N., Ek, O., Peers, B., and Meyer, D., Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish, 290-302, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.