Fig. S5

Fig. S5

gle1 morphants have an earlier onset of phenotypes than gle1^-/- mutants. (A-D) Embryos from gle1^hi4161a/+ intercross were injected with a mix of two non-overlapping gle1 translation-blocking morpholinos (gle1 MOs, C,D) or with five-nucleotide-mismatched MOs (5mm gle1 MOs) (control, A,B). At 30 hpf, cell death can be seen in the CNS of the gle1 MO-injected morphants (bright field, C; Acridine Orange stained, D), but not in the embryos injected with the 5mm gle1 MOs (bright field, A; Acridine Orange stained, B). (E,F) Motoneuron development was examined by confocal microscopy in embryos from gle1^hi4161a/+ intercross injected with either the gle1 MOs (F) or the 5mm gle1 MOs (E) as described in A-D. At 25 hpf, the gle1 MO-injected embryo (F) develops fewer motoneurons in the spinal cord than the 5mm gle1 MO-injected embryo (E). However, motor axon outgrowth in the gle1 MO-injected embryo still appears normal as compared with the control (E) at this stage. All images are lateral views of the spinal cord. Motoneurons (E,F) were imaged at somites 12-15 above the yolk extension with dorsal to top, anterior to left. Scale bars: 500 μm in D; 25 μm in F.