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Fig. 7
Gle1 acts non-cell-autonomously in motor axon arborization. (A-C3) Confocal images of live chimera embryos at 3 dpf. Cells from either wild-type (A,B) or gle1 morphant (C) donor embryos (mnx1:GFP+) were transplanted into wild-type (A,C) or gle1 morphant (B) hosts (mnx1:TagRFP-T+). Wild-type donor motoneurons extend their axons and follow the aberrant motor axons of the gle1 morphant host (B). Conversely, motoneurons from the gle1 morphant donor extend their axons normally in the wild-type host (C) as in the case with the wild type-to-wild type transplantation (A). Rostrally projecting motor nerves from the gle1 morphant donor are restored in the wild-type environment (asterisks, C). Single-channel images (A2,A3,B2,B3,C2,C3) are shown beneath the merged images to distinctly visualize donor and host axons. Images are lateral views of the trunk spinal cord with dorsal to top, anterior to left. Scale bars: 75 μm.