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Fig. S1 Confirmation of specific activities of arl6ip1-MO1 in zebrafish embryos. (A) Injection of 4 ng arl6ip1-MO1 caused brain lesions and foreshortened trunks. The defects of arl6ip1-MO1 were rescued either partially by the wobble arl6ip1 mRNA (E) and wobble arl6ip1-gfp mRNA (B) or almost completely by wobble arl6ip1-gfp mRNA with p-53 MO (C). (F, G) By detecting the GFP signals, we confirmed that the arl6ip1-MO1 cannot target wobble arl6ip1-gfp mRNA. (D) The Arl6ip1-GFP fusion protein was detected at 24 hpf in embryos injected with arl6ip1-MO1-target-gfp mRNA. (H) The GFP signal was absent at 24 hpf in embryos co-injected with arl6ip1-MO1-target-gfp mRNA and arl6ip1-MO1. (I) The arl6ip1 morphants did not display sulcus and gyrus in brains, and these brain defects were rescued either partially by wobble arl6ip1-gfp mRNA (J) or completely by wobble arl6ip1-gfp mRNA with p53-MO (K).