ZFIN Image: Xu et al., 2012, Fig. S2

Image

Figure Caption

Fig. S2 nanog-like morphants can express ndr1 and ndr2 upon receiving Nodal signals, related to Figure 2

(A) 4-cell stage embryos were injected with 20pg GFP mRNA at the vegetal pole of the yolk cell and imaged at the dome stage. Embryos were classified based on their GFP expression pattern.

(B) 107 GFP mRNA-injected embryos were sorted into three categories based on their GFP expression pattern.

(C) 1-cell stage embryos were injected with 10ng control morpholino (con-MO), or 10ng nanog-like-MO1. To activate the Nodal signaling, 100pg Tar* mRNA was co-injected. Expression of ndr1 and ndr2 was examined by whole mount in situ hybridization.

Acknowledgments

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Reprinted from Developmental Cell, 22(3), Xu, C., Fan, Z.P., Müller, P., Fogley, R., Dibiase, A., Trompouki, E., Unternaehrer, J., Xiong, F., Torregroza, I., Evans, T., Megason, S.G., Daley, G.Q., Schier, A.F., Young, R.A., and Zon, L.I., Nanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway, 625-238, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell